Expression Pattern of the Driver Line TPH-GAL4 in the Larval CNS.

<p>First column: CNS of TPH-GAL4/UAS-mCD8::GFP third instar larvae stained with anti-CD8 (green), anti-5HT (red) and anti-FasII/anti-ChAT (neuropil markers; blue). The second, third and fourth columns represent the three channels separately. The first row (A–A’’’) shows an overview of the CNS. Other rows represent higher magnifications of the brain in frontal view with slightly shifted brain hemispheres (B–B’’: posterior; C–C’’: anterior) and the ventral nerve cord (VNC) (D–D’’). In the posterior (B–B’’) and anterior brain (C–C’’) as well as in the VNC (D–D’’) most cells are both anti-CD8 and anti-5HT positive. Only in some clusters (e.g. SE3 and T1) a few CD8-positive cells do not show 5HT expression. Details are also presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047518#pone-0047518-t001" target="_blank">Table 1</a>. Scale bars: 50 µm.