<p>Sequences of the primers used in this study, mutated sites are in bold type.</p
<p>Restriction enzyme sites generated by site-directed mutagenesis or by PCR are boldfaced and under...
<p>*The restriction sites in the sequences are indicated by lower cases. The corresponding restricti...
<p>Wt: Wild-type; Mut: mutant; F: Forward primer; R: Reverse primer. The sequences of restriction si...
<p>*The bold and underlined letters denote the mutated sequences.</p><p>Primers used for site-direct...
<p>Underlined sequences indicate restriction enzyme sites. Bold indicates mutated sequences.</p
<p>Lower case sequences added to obtain appropriate length. Bold indicates a mutation or restriction...
<p>Primers used in this study. Restriction sites are underlined and changed codons are in bold.</p
1<p>F, forward primer; R, reverse primer.</p>2<p>The bases changed are shown in boldface type in eac...
<p>Primer sequences of the 6 primers used and the mutation frequency detected.</p
Primers used in this study(underlined in the primer sequences were restriction enzyme sites).</p
<p>Note: Italic and bold indicate mutation base.</p><p>The summary of the primers used in this study...
<p>The sequences of primers used in this study, the restriction enzyme sites used in the study are u...
<p>*underlined regions are mutated bases</p><p>List of primers used in the study.</p
<p>Note: <i>Nco</i>I and <i>Hind</i>III restriction sites were marked in single underline; the mutat...
a<p>Restriction sites are underlined in the primer sequence.</p><p>Primer used in this study.</p
<p>Restriction enzyme sites generated by site-directed mutagenesis or by PCR are boldfaced and under...
<p>*The restriction sites in the sequences are indicated by lower cases. The corresponding restricti...
<p>Wt: Wild-type; Mut: mutant; F: Forward primer; R: Reverse primer. The sequences of restriction si...
<p>*The bold and underlined letters denote the mutated sequences.</p><p>Primers used for site-direct...
<p>Underlined sequences indicate restriction enzyme sites. Bold indicates mutated sequences.</p
<p>Lower case sequences added to obtain appropriate length. Bold indicates a mutation or restriction...
<p>Primers used in this study. Restriction sites are underlined and changed codons are in bold.</p
1<p>F, forward primer; R, reverse primer.</p>2<p>The bases changed are shown in boldface type in eac...
<p>Primer sequences of the 6 primers used and the mutation frequency detected.</p
Primers used in this study(underlined in the primer sequences were restriction enzyme sites).</p
<p>Note: Italic and bold indicate mutation base.</p><p>The summary of the primers used in this study...
<p>The sequences of primers used in this study, the restriction enzyme sites used in the study are u...
<p>*underlined regions are mutated bases</p><p>List of primers used in the study.</p
<p>Note: <i>Nco</i>I and <i>Hind</i>III restriction sites were marked in single underline; the mutat...
a<p>Restriction sites are underlined in the primer sequence.</p><p>Primer used in this study.</p
<p>Restriction enzyme sites generated by site-directed mutagenesis or by PCR are boldfaced and under...
<p>*The restriction sites in the sequences are indicated by lower cases. The corresponding restricti...
<p>Wt: Wild-type; Mut: mutant; F: Forward primer; R: Reverse primer. The sequences of restriction si...
DOI
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