Spironolactone partially normalizes mRNA expression of Dot1a, Af9, and ET-1 in STZ-induced diabetic rats. (A)

<p>As in Fig. 1, representative agarose gel analyses of RT-PCR for expression of genes as indicated in the cortex of rats. (<b>B–D</b>) For each mRNA as indicated, the intensity of each corresponding band was quantified using ImageJ64, normalized to that of GAPDH or β-actin as shown in <b>A</b>, averaged within each group, and plotted, with the relative intensity in control (CT) set to 1. n = 8 rats/group. *P<0.05 vs. CT. #P<0.05 vs. STZ.