Plasminogen bound to Sbi or Efb is converted to plasmin.

<p>(A) Plasmin (PL) bound to Sbi, Efb, and to the borrelial CRASP-5 converted a chromogenic substrate as followed by measuring absorbance at 405 nm after 24 h. Equimolar amounts of bacterial proteins were immobilized and incubated with plasminogen (PLG). After washing, the activator uPA was applied together with the chromogenic substrate S-2251. Human serum albumin (HSA) had no effect. (B+C) Plasmin generation of plasminogen bound to Sbi (B) and Efb (C) by uPa or staphylokinase (SAK). The plasmin activators uPa and SAK or no activator (w/o) were applied to plasminogen bound to Sbi or Efb together with the chromogenic substrate. Conversion of the chromogenic substrate was determined at various time points. Data represent mean values ± standard deviations from three independent experiments.