IL-33 mediates a MC-FLS amplification loop.

<p>MCs were cultured with or without FLS for 1–2 weeks in the upper and lower chambers, respectively, of a transwell apparatus. (A) Cytokine mRNA expression in WT and ST2^−/− MCs after co-culture with FLS. (B) Cytokine mRNA levels in MCs with or without anti-IL-33 Ab treatment (10 µg/ml q4d). Data represent 2 independent experiments with similar results. (C–D) Cytokine mRNA levels in FLS. n = 2 wells per condition, reflective of 2–5 pooled experiments. *<i>P</i><0.05, **<i>P</i><0.01, N.S., not significant.