TCR dependent proliferation and apoptosis are unaltered in total thymocytes of LynΔN mice.

<p><b>A)</b> Apoptosis index determined by TUNEL staining (green staining) was analyzed onto frozen thymi sections of 2-week old LynΔN and control mice. <b>B)</b> AnnexinV positive cells were analyzed onto freshly isolated thymocytes from control and LynΔN transgenic mice (n = 6 in each group) by flow cytometry. <b>C)</b> Total thymocytes from LynΔN and control mice were stimulated or not with 10 µg/ml of plate-coated anti-CD3 for the indicated time periods and AnnexinV+/PI+ dead cells were determined by flow cytometry. <b>D)</b> Total thymocytes from LynΔN and control mice were stimulated with 10 µg/ml of plate-coated anti-CD3 plus soluble anti-CD28 (2 µg/ml) mAbs for 72 h. 10 µM BrdU was added for 16 hours, and proliferation was measured by BrdU incorporation. Results are expressed as mean ± SD. Data are representative of at least 3 independent experiments.