Characterization of TGFβ1-induced myoFB.

<p>Confluent myoFB were exposed to NGF at the indicated doses/times and evaluated for biochemical and molecular changes. (A) αSMA protein expression in both myoFB and FB, as detected by cell surface ELISA (optic density) and flow cytometry (fluorescence intensity). (B–C) Fluorescent histograms showing a different pattern of trkA^NGFR and p75^NTR expression in acute and chronic 100 ng/mL NGF exposed myoFB, as compared to untreated myoFB (black line). Mean Fluorescent Intensity (MFI) plots are shown inside fluorescent histograms (iso stands for isotype-matched control FI; 7.05 for trkA^NGFR and 8.39 for p75^NTR). (D) Morphological distribution of trkA^NGFR/p75^NTR in untreated, acute and chronic 100 ng/mL NGF treated myoFB. (E–F) Target gene expression specific for trkA^NGFR/p75^NTR after acute and for αSMA/p75^NTR after chronic exposure to increasing NGF (p<.05).