Effect of TNF on expression of mitochondrial respiratory complexes I, II and III measured by EPR spectroscopy.

<p>The activities of mitochondrial respiratory a) complex I, b) complex II, and c) complex III were assessed using EPR spectroscopy. TNF decreased the activities of all three complexes in isolated heart mitochondria; these results indicate a derangement in electron transport chain activity. Losartan attenuated these changes. * p<0.05 vs. control; $ p<0.05 vs. TNF d) ATP production rates and e) ATP/ADP ratios of heart mitochondria from each experimental group. TNF administration resulted in significant decreases in both ATP production rate and ATP/ADP ratio, which is suggestive of electron transport chain dysfunction. Losartan attenuated the changes seen in ATP production and ATP/ADP ratio. * p<0.05 vs. control; $ p<0.05 vs.TNF.