Effect of CSA on phosphorylation of MLC, MYPT1, and PKC.

<p>Effect of 10 µM CSA on (A and B) U46619- or (C, D and E) PMA-induced phosphorylation of MLC at Ser19 (p-MLC), MYPT1 at Thr853 (p-MYPT1) and PKCδ at Thr505 (p-PKCδ) as compared to their total levels (t-MLC, t-MYPT1 and t-PKCδ). The lower bands in the t-MYPT1 blot in (B) were unknown proteins probably non-specifically probed by the primary antibodies. Values are means ± S.E.M of 8 experiments. *P<0.05 compared with control and #p<0.05 compared with U46619 or PMA.