Treatment with B-DIM or metformin inhibits prostasphere-forming ability.

<p>C4-2B cells were treated with indicated doses of B-DIM (A, B) for 6 days. Treatment with different concentrations of metformin served as controls (C, D). After 6 days, prostasphere numbers were counted under the microscope and the proportion of sphere-generating cells was calculated by dividing the number of cells seeded by the number of prostaspheres (A, C). Prostaspheres from C4-2B cells treated with B-DIM (B) or metformin (D) were photographed and the results showed that 10 and 25 µM of B-DIM significantly reduced size and numbers of prostaspheres. n = 6, * P<0.05, ** P<0.01.