Characterization of peripheral eNANC airway responses in guinea pigs.

<p><b>A.</b> Capsaicin was added to precision-cut lung slices (PCLS) at a concentration of 10 µM, either after 15 min pre-incubation with an unspecific transient receptor potential (TRP) channel antagonist (ruthenium red, SKF96365) or without pre-incubation (control). Data are shown as mean – SEM; n = 5 PCLS from 5 guinea pigs. 100% of the initial airway area (IAA) was considered as baseline and the area above the curve (AAC), i.e., the area enclosed by the baseline and the curve, is depicted as shading. Statistical analysis was performed on the AAC; *, p<0.05 in ANOVA followed by the Tukey test. <b>B.</b> Electric field stimulation (EFS; F = 50 Hz, B = 1 ms, A = 200 mA, SD = 2.5 s) was carried out on PCLS either after pre-incubation with TRP channel blockers or without pre-treatment (control). The minimal airway area obtained in the EFS of PCLS was used in statistical analysis showing an effect for both TRP channel antagonists. Data are shown as mean - SEM; n = 6 PCLS from 6 guinea pigs; *, p<0.05 and ^##, p<0.01 for the comparison of the control group to the ruthenium red and SKF96365 group, respectively, in the Tukey multiple comparison test.