<p>MLR induced by non-sorted, CD1a+, and CD1a−negative DC purified at day 3 of monocyte differentiation (immature DC, iDC) and cultured an additional 48 h in the presence of LPS at 10 ng/ml (mature DC, mDC). The percentage of proliferation of CFSE-labeled T cells was determined as described in Methods. Patients, filled bars; controls, opened bars. Numbers (n) in each group are indicated. Histograms represent mean ± SD. § p<0.05 Wilcoxon non parametric test between iDC and mDC, 2 p<0.05 Wilcoxon non parametric test between non-sorted and either CD1a+ or CD1a−negative mDC.</p
<p>A) CFDA-labeled PBMC were incubated with PBS (CNT), MOi or Con A [5 µM] for seven days. CFDA<sup>...
<p>The extent of proliferation was calculated after 72-culture from the decrease in fluorescence per...
Inhibition of T-cell proliferation is the most common approach to assess human myeloid-derived suppr...
<p>MLR was induced by CD1a+ and CD1a−negative DC purified at day 3 of monocyte differentiation (imma...
<p>Flow cytometry analysis of HLA-DR in monocytes, and in CD1a+ and CD1a−negative DC purified at day...
<p>IL-10 (A) and IL-12p40 (B) levels were assayed in culture supernatants of CD1a+ and CD1a−negative...
For an allogeneic MLR, responder cells from 6 healthy donors were stained with CFSE and mixed with γ...
<p>DCs were differentiated and matured (IFNγ/LPS) in RPMI medium in the presence of GM-CSF and IL-4,...
<p>Purified pre-RTEs (SP4 thymocytes) and naïve CD4<sup>+</sup> T cells were labeled with CFSE and c...
The round THP-1 cells changed to be adherent flatten cells (white arrows). Under forward scatter and...
<p><b>A.</b> β<sub>1</sub>-AA promoted CD3<sup>+</sup>T cell proliferation in a concentration-depend...
<p><b>A.</b> An allogeneic mixed lymphocyte reaction (MLR) was used to determine if DCs (stimulator)...
<p>Dot plots represent fluorescence of lymphocytes identified by forward and side scatter after co-c...
(A). DCs were activated with Echinococcus granulosus antigens and cocultured in graded amounts with ...
<p>Immature lowGM-DC or mature highGM/4-DC were generated to stimulate allogeneic naïve T cells. Aft...
<p>A) CFDA-labeled PBMC were incubated with PBS (CNT), MOi or Con A [5 µM] for seven days. CFDA<sup>...
<p>The extent of proliferation was calculated after 72-culture from the decrease in fluorescence per...
Inhibition of T-cell proliferation is the most common approach to assess human myeloid-derived suppr...
<p>MLR was induced by CD1a+ and CD1a−negative DC purified at day 3 of monocyte differentiation (imma...
<p>Flow cytometry analysis of HLA-DR in monocytes, and in CD1a+ and CD1a−negative DC purified at day...
<p>IL-10 (A) and IL-12p40 (B) levels were assayed in culture supernatants of CD1a+ and CD1a−negative...
For an allogeneic MLR, responder cells from 6 healthy donors were stained with CFSE and mixed with γ...
<p>DCs were differentiated and matured (IFNγ/LPS) in RPMI medium in the presence of GM-CSF and IL-4,...
<p>Purified pre-RTEs (SP4 thymocytes) and naïve CD4<sup>+</sup> T cells were labeled with CFSE and c...
The round THP-1 cells changed to be adherent flatten cells (white arrows). Under forward scatter and...
<p><b>A.</b> β<sub>1</sub>-AA promoted CD3<sup>+</sup>T cell proliferation in a concentration-depend...
<p><b>A.</b> An allogeneic mixed lymphocyte reaction (MLR) was used to determine if DCs (stimulator)...
<p>Dot plots represent fluorescence of lymphocytes identified by forward and side scatter after co-c...
(A). DCs were activated with Echinococcus granulosus antigens and cocultured in graded amounts with ...
<p>Immature lowGM-DC or mature highGM/4-DC were generated to stimulate allogeneic naïve T cells. Aft...
<p>A) CFDA-labeled PBMC were incubated with PBS (CNT), MOi or Con A [5 µM] for seven days. CFDA<sup>...
<p>The extent of proliferation was calculated after 72-culture from the decrease in fluorescence per...
Inhibition of T-cell proliferation is the most common approach to assess human myeloid-derived suppr...