Cytokine production induced by DC derived from septic patients in MLR.

<p>MLR was induced by CD1a+ and CD1a−negative DC purified at day 3 of monocyte differentiation (immature DC, iDC) and cultured an additional 48 h in the presence of LPS at 10 ng/ml (mature DC, mDC). Concentrations of IFN-γ (A), IL-10 (B), IL-4 (C), and TGF-β (D) were assayed in cultured supernatants and expressed in picograms per millions T cells initially present in cultures. Results are shown as individual values with median for each group. Patients, filled circles (n = 6); controls, opened circles (n = 6 except CD1a−negative iDC and mDC n = 5). § p<0.05 Wilcoxon non parametric test between iDC and mDC.