<p>Cells were sorted at day 3 of monocyte differentiation, put back in culture for 4 additional days (iDC), and matured for 2 days with 10 ng/ml LPS (mDC).</p><p>Minimum and maximum fluorescence values (number of sites per cell) of each group in each cell type were determined and results were scored as follows:</p><p>0 site per cell: 0; low to 5 000 sites: ±; 5 000 to 10 000 sites: +; 10 000 to 100 000 sites: ++; >100 000 sites: +++.</p
<p>DC subtypes were identified for cell surface molecule analyses as shown. An example of the leukoc...
<p>(A) Expression of surface markers CD14, CD19, CD34, CD45, HLA-DR, CD73, CD90, CD105 (filled) with...
<p>Peripheral blood mononuclear cells (PBMC) and granulocytes were obtained by density gradient cent...
Fluorescent immunophenotyping uses fluorescently-conjugated antibodies to identify, characterize and...
<p>(<b>A</b>) Digested tissue samples were subjected to PI live/dead cell exclusion and CD45<sup>+</...
<p>Cells were stained (A) with fluorescently labelled antibodies against cell surface Fcγ receptors ...
<p>(A) FSC x SSC gating to obtain mononuclear cells based on size and granularity. (B) Live/dead Aqu...
<p>PBMC were stained with mouse monoclonal antibodies and then analyzed by flow cytometry. Monocytes...
Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex...
<p>Expression of cellular membrane antigen markers on day 0 and after 10 days of culture in presence...
<div><p>Flow cytometry is the primary immunological technique used to analyze multiple parameters on...
<p>Aliquots of fresh, live peripheral blood mononuclear cells (PBMNC) were stained with anti-CD4-all...
<p>Significantly increased (*; p≤0.05) expression of A) CD1a and B) CD14 and up-regulation of co-sti...
<p>(A) Shown are isotype control dot plots and CD14 and CD16 expression in freshly obtained and cryo...
Context: Simple tandem repeat loci are used to track bone marrow engraftment using mononuclear buffy...
<p>DC subtypes were identified for cell surface molecule analyses as shown. An example of the leukoc...
<p>(A) Expression of surface markers CD14, CD19, CD34, CD45, HLA-DR, CD73, CD90, CD105 (filled) with...
<p>Peripheral blood mononuclear cells (PBMC) and granulocytes were obtained by density gradient cent...
Fluorescent immunophenotyping uses fluorescently-conjugated antibodies to identify, characterize and...
<p>(<b>A</b>) Digested tissue samples were subjected to PI live/dead cell exclusion and CD45<sup>+</...
<p>Cells were stained (A) with fluorescently labelled antibodies against cell surface Fcγ receptors ...
<p>(A) FSC x SSC gating to obtain mononuclear cells based on size and granularity. (B) Live/dead Aqu...
<p>PBMC were stained with mouse monoclonal antibodies and then analyzed by flow cytometry. Monocytes...
Flow cytometry is the primary immunological technique used to analyze multiple parameters on complex...
<p>Expression of cellular membrane antigen markers on day 0 and after 10 days of culture in presence...
<div><p>Flow cytometry is the primary immunological technique used to analyze multiple parameters on...
<p>Aliquots of fresh, live peripheral blood mononuclear cells (PBMNC) were stained with anti-CD4-all...
<p>Significantly increased (*; p≤0.05) expression of A) CD1a and B) CD14 and up-regulation of co-sti...
<p>(A) Shown are isotype control dot plots and CD14 and CD16 expression in freshly obtained and cryo...
Context: Simple tandem repeat loci are used to track bone marrow engraftment using mononuclear buffy...
<p>DC subtypes were identified for cell surface molecule analyses as shown. An example of the leukoc...
<p>(A) Expression of surface markers CD14, CD19, CD34, CD45, HLA-DR, CD73, CD90, CD105 (filled) with...
<p>Peripheral blood mononuclear cells (PBMC) and granulocytes were obtained by density gradient cent...