FlAsH detection of ExoT-Cys₄ in an aggregate of the mucoid isolate FRD1.

<p>Phase contrast images of (A) PAO1, (B) FRD1 and (C) FRD2 mid-log planktonic cultures. Fluorescence microscopy shows a mid-log culture of mucoid FRD1 stained with (D) FlAsH to detect the production of ExoT-Cys₄ and (E) a membrane-specific dye FM 4–64 (red). The merged image is shown in (F). Each experiment was performed at least three times and representative images are shown.