Redox, acetyl CoA and cholesterol levels in G6PD^deficient mice hearts.

<p>Metabolites produced from NADPH-dependent reaction were estimated in wild-type and G6PD^deficient mice hearts. A and B: G6PD deficiency decreased reduced glutathione-to-oxidized glutathione (GSH-to-GSSG; A) ratios determined by colorimetric assay and superoxide (O2−; B) estimated by lucigenin (5 µM) chemiluminescence method. Concurrently, G6PD deficiency lowered NADPH-dependent free fatty acid β-oxidation, which produces acetyl CoA (C), cholesterol synthesis (D), and caveolin (E–F) in the hearts as well.