Northern blot analysis of TNF-α mRNA expression in the RWAT of lean and <i>fa/fa</i> rats.

<p>Northern hybridization of TNF-α mRNA and 18S ribosomal RNA (as control) was performed using the standard protocol. Briefly, total RNA from retroperitoneal WAT was extracted by Chomczynski method (Chomczynski 1993). Total RNA (∼20 µg) was denatured and separated on formaldehyde gel. Lane 1, L0, lean-pretreatment at week 0; lanes 2 and 3, S-lean; lanes 4 and 5, CL-lean; lane 6, F0, <i>fa/fa</i>-pretreatment at week 0; lanes 7 and 8, S-<i>fa/fa</i> and lanes 9 and 10, CL-<i>fa/fa</i> rats.