2-CDA increases Aβ and CTF levels in CHO cells stably expressing WT APP751 (7WD10 cells).

<p>(A) 7WD10 cells were exposed to different concentrations of 2-CDA for 48 h and the conditioned medium was immunoprecipitated for Aβ and detected by immunoblots using NuPAGE 4–12% bis-tris gel. The lysates were used to detect c-terminal fragments (CTFs) using NuPAGE gels. APP as well as actin as a loading control were separated using 10% bis-acrylamide gels. (B) Quantitation by image J revealed increased levels of Aβ at 1.0 µM (69% (p<0.01), 5.0 µM (84%, p<0.01) and 10.0 µM (107%, p<0.01) compared to controls. Values are expressed as percentage change from controls. CTF levels were also significantly increased at 0.5 µM (278%, p<0.05), 1.0 µM (302%, p<0.01), 5.0 µM (302%, p<0.01) and 10.0 µM (301%, p<0.01) compared to controls. For all samples, n = 4. *, p<0.05, **, p<0.01, versus control by analysis of variance (ANOVA) followed by post-hoc test by Dunnett multiple comparisons.