2-CDA increases sAPPβ levels but not sAPPα or sAPPtotal.

<p>(A) 7WD10 cells were treated with different concentrations of 2-CDA as indicated and after 48 h the conditioned medium was subjected to immunoblotting to detect different species of sAPPs. (B) sAPPβ levels were increased to 250% at 0.1 µM, 375% (p<0.05) at 0.5 µM, 484% (p<0.05) at 1.0 µM, 518% at 5.0 µM and 378% at 10.0 µM compared to untreated cells. (C) sAPPα levels were not significantly altered in 2-CDA treated cells versus controls at any of the concentrations. (D) Levels of sAPPtotal were also not altered in 2-CDA treated cells. For all samples, n = 4. *, p<0.05 versus controls by repeated measures ANOVA followed by Dunnett multiple comparison test.