Receptor status and activation of ERα, ERβ and GPER in MCF-12A and MCF-10A cells.

<p><b>A</b>. ERα, ERβ and GPER expression detected by immunoblotting. <b>B</b>. Regulation of ER-target genes. White bars represent treatment with 1 nM E2. Hatched white bars show the effects of E2 in combination with ICI 182,780 (1 µM). Horizontal dashed line corresponds to the negative controls (0.5% ethanol), set to 1. Data are from 3 independent experiments and error bars are mean ± SEM. **a) shows statistically significant differences (p&lt;0.01) between treatments and controls. **b) (p&lt;0.01) and *b) (p&lt;0.05) highlight significant differences between treatments with E2 tested individually and treatments with E2 combined with ICI 182,780.