Proteolytic activity of aureolysin and elastase on peptides M33-L and M33-D.

<p><b>a</b> and <b>b</b>, HPLC and MS profiles, respectively, of M33-L before incubation with enzymes. <b>c</b> and <b>d</b>, HPLC and MS profiles, respectively, of M33-D before incubation with enzymes. In HPLC the retention time of M33-L and M33-D was 23 minutes. The calculated MW of M33 was 4682. <b>e</b> and <b>f</b>, HPLC and MS, respectively, of M33-L incubated for 1 hour with aureolysin. <b>f</b> shows the peaks indicating the proteolytic site (RL or SA). <b>g</b> and <b>h</b>, HPLC and MS, respectively, of M33-D incubated for 24 hours with aureolysin. <b>i</b> and <b>j</b>, HPLC and MS, respectively, of M33-L incubated for 5 hours with elastase. <b>j</b> shows the peaks indicating the proteolytic site (RL or SA). <b>k</b> and <b>l</b>, HPLC and MS, respectively, of M33-D incubated for 24 hours with elastase. <b>m</b>, proteolytic sites of the two enzymes on the tetrabranched M33 are indicated by arrows. The table assigns MS peaks to the cleavage fragments.