Liver protection against acute liver failure following GSK3β inhibition is dependent of regulating inflammatory reaction and modulating the MAP kinases signaling.

<p>(a) The gene expression levels of inflammatory IL-12p40, IL-10 at 2 hour after D-GalN/LPS injection, and TNF-α, IL-1β and IL-6 at 6 h after D-GalN/LPS injection(n = 5–6/group). (b) Liver MPO levels at 6 h after D-GalN/LPS injection (n = 5–6/group). (c) The phosphorylated MAP kinases including JNK, ERK and p38, and β-actin were measured by Western blots. Liver samples were harvested from C57BL/6 mice that were subjected to PBS (Control, n = 5–6) or D-GalN/LPS, followed by various times (1, 3, and 6 h, n = 5–6/group). Representative of two experiments is shown. Densitometry analysis of the proteins was performed for each sample (mean±SEM). (d) The MAP kinases are regulated by GSK3β inhibition in SB216763-pretreated acute liver failure in mice. Groups of C57BL/6 mice were treated with vehicle (DMSO, n = 5–6/group), or SB216763 (50 mg/kg, n = 5–6/group), followed by 6 h D-GalN/LPS injection. Representative of two experiments is shown. Densitometry analysis of the proteins was performed for each sample (mean±SEM).