The Inhibition of GSK3β prevents ERS-induced hepatocyte apoptosis <i>in vitro</i> and <i>in vivo</i>.

<p>(a) LDH activities assay in the hepatocyte culture supernatant. SB216763 was added 2 h before tunicamycin(10 µg/ml, Sigma) which is added into the 24 wells culture plate for 24 hours(n = 3 wells). (b) The hepatocyte proteins expression of caspas-3, cleaved caspase-3 and β-actin were measured by Western blots. SB216763 was added 2 h before tunicamycin(10 µg/ml, Sigma) which was added into the 60 cm culture well for 12 hours, total lysates were subjected to immunoblot assay, and densitometry analysis(mean±SEM) was performed (n = 3). (c) The liver proteins expression of ERS (GRP78, CHOP, caspase-12) were measured by Western blots. Liver samples were harvested from C57BL/6 mice at 6 h after D-GalN/LPS injection that were pretreated with only PBS (Control, n = 5–6), and vehicle (DMSO, n = 5–6/group), or SB216763 (50 mg/kg, n = 5–6/group) before D-GalN/LPS injection. Representative of two experiments is shown. Densitometry analysis of the proteins was performed for each sample (mean±SEM).