Regulation of Shh pathway by SFN in pancreatic CSCs <i>in vitro</i>.

<p>(<b>A–C</b>), Inhibition of components of sonic hedgehog pathway. Pancreatic CSCs were treated with sulforaphane (0–20 µM) for 24 h. The expression of Gli1, Gli2 and Smo was measured by qRT-PCR and normalized to GAPDH expression. All assays were performed in triplicate and were calculated on the basis of ΔΔ<i>C</i>t method. Data represent mean ± SD. *, @, and $ = significantly different from control, P < 0.05; (<b>D</b>), Immunoblotting of Gli1/2, Smo and β-actin of human pancreatic cancer stem cells (PanCSC) treated with Sulforaphane (0–20 µM).