Complementation of mitofusin-2 KO cells.

<p>Mfn2 KO cells were transfected with a plasmid driving the expression of Mitofusin-2 fused to GFP. Cells expressing moderate levels of fluorescence were sorted, re-grown, fixed and processed as described in the legend to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0046293#pone-0046293-g001" target="_blank">Fig. 1</a>. For each individual cell profile, the frequency of ER-mitochondria tethering (expressed as % of the total mitochondrial perimeter) was determined, as well as the average width of ER cisternae. Mock-transfected <i>mfn2</i> KO cells (red circles) exhibited wider ER cisternae and more ER-mitochondria tethering than cells re-expressing Mitofusin-2 (green triangles). For each population of cells, the average and S.E.M. are indicated in black.