Effects of EGCG on NO-induced apoptosis-related genes in HEI-COΙ cells.

<p>(A) Cells were pretreated with 50 μM EGCG, followed by treatment with 500 μM SNAP for 24 h. After isolation of cytosolic and mitochondrial fractions, the protein extracts were assayed for cyt <i>c</i> by western blot analysis. GAPDH was used as an internal cytosolic marker control, and VDAC was used as a mitochondrial marker. (B) The relative levels of cytosolic and mitochondrial cyt <i>c</i> were quantified by densitometry. (C) Western blot analysis revealed a reduction in Bcl-2 protein after SNAP exposure. (D) Relative levels of Bcl-2 are shown. (E) The levels of cleaved caspase-3 after treatment with EGCG were assayed by western blot analysis. (F) The effect of EGCG on caspase-3 activation was determined using a colorimetric kit. All data represent the mean ± SEM of 3 independent experiments (<i>^#P</i><0.05 vs. control, *<i>P</i><0.05 vs. SNAP alone).