Genipin treatment up-regulates the expression of TIMP-1 in HCC cells.

<p><b>A</b> shows the quantitative real-time PCR screening of endogenous MMP-2/9 inhibitors. Cells were treated with genipin for 24H and total RNA was collected for anlaysis. The result shows that genipin particularly up-regulate TIMP-1 mRNA transcription but has no effect on TIMP-2, -3, -4 and RECK mRNA transcript in HepG2 and MHCC97L cells. <b>B</b> shows that genipin exposure is responsible for the increased secretion of TIMP-1 in HepG2 and MHCC97L cells. The cells were transfected with scr negative control siRNA or siRNA against TIMP-1. Then cells were treated with genipin for 24H then medium was collected for ELISA determination of TIMP-1. *p<0.05,p<0.01 when compared with control; <b>C</b> shows that genipin up-regulates expression of TIMP-1. The cells were transfected with scr negative control siRNA or siRNA against TIMP-1. Then cells were treated with genipin for 24H and cell lysates were analyzed with immunoblotting.