Characterization of cells in co-culture consisting of human primary trophoblasts, DSCs and DICs of early human pregnancy.

<p>In the co-culture, the attached trophoblasts (A) and DSCs (B) were stained and recognized by CK7 and vimentin antibodies, resulting in specific brown staining in the membrane and cytoplasm, respectively. No specific staining was seen with the isotype-control (C). Immune cells in suspension were harvested and stained with CD45-FITC (D) or the isotype control (E), and then subjected to flow cytometry. The percentages of positive cells in the indicated cells were shown. Data represent the mean ± SE of three independent experiments with 14 villi and three deciduas, performed in triplicate wells with three different samples.