Mchr1 localizes to neuronal cilia throughout the mouse brain.

<p>(A–L) Representative images of multiple brain regions in 5–6 week old WT mice showing colabeling for Mchr1 (green) and AC3 (red). Nuclei are stained with DRAQ5 (blue). Labeling for AC3 reveals numerous primary cilia present throughout the CA1 region of the hippocampus (B), amygdala (E), piriform cortex (H), and fasciolar gyrus (K). Labeling for Mchr1 (A, D, G, & J) reveals abundant Mchr1 ciliary localization in each region. Merged images (C, F, I, L) showing colocalization between Mchr1 and AC3. Scale bars represent 10 µm.