Mchr1 and Sstr3 colocalize in a subset of piriform cortical neuronal cilia.

<p>(A–F) Representative image of the piriform cortex from an adult WT mouse colabeled for Mchr1 (green) and Sstr3 (red). Nuclei are stained with DRAQ5 (blue). Labeling for Mchr1 (A) and Sstr3 (B) reveals the presence of Mchr1- and Sstr3-positive cilia in the piriform cortex. Merged image (C) shows colocalization of Mchr1 and Sstr3 on a subset of neuronal cilia. Zoomed in image (D–F) reveals a subset of neuronal cilia that are positive for both Mchr1 and Sstr3 (arrowhead). (G) Quantification of day 7 WT piriform cortical neurons colabeled for Mchr1 and AC3 or Sstr3 and AC3 reveals that Mchr1 localizes to 55.62±3.63% (n = 186) of AC3-positive cilia and Sstr3 localizes to 14.03±1.71% (n = 159) of AC3-positive cilia. (H) Graphical representation of the quantification of day 7 WT piriform cortical neurons colabeled for Mchr1 and Sstr3 shows Sstr3 colocalizes to 23.36±5.11% (n = 86) of Mchr1-positive cilia. Scale bars represent 10 µm.