Mchr1 and Sstr3 colocalize in a subset of hypothalamic neuronal cilia.

<p>(A–F) Representative image of the hypothalamus from an adult WT mouse colabeled for Mchr1 (green) and Sstr3 (red). Nuclei are stained with DRAQ5 (blue). Labeling for Mchr1 (A) and Sstr3 (B) reveals the presence of Mchr1- and Sstr3-positive cilia in the hypothalamus. Merged image (C) shows colocalization of Mchr1 and Sstr3 on a subset of neuronal cilia. Zoomed in image (D–F) reveals a subset of neuronal cilia that are positive for both Mchr1 and Sstr3 (arrowhead). (G) Quantification of day 7 WT hypothalamic neurons colabeled for Mchr1 and AC3 or Sstr3 and AC3 reveals that Mchr1localizes to 63.42±4.59% (n = 108) of AC3-positive cilia and Sstr3 localizes to 33.34±5.24% (n = 129) of AC3-positive cilia. (H) Graphical representation of the quantification of day 7 WT piriform cortex neurons colabeled for Mchr1 and Sstr3 shows Sstr3 colocalizes to 20.36±4.39% (n = 85) of Mchr1-positive cilia. Scale bars represent 10 µm.