Arf4^+/− mice are not impaired in spatial learning and memory, general locomotor activity, motor coordination, or anxiety behavior.

<p>Mice were 4–5 months of age at the time of experimentation (12–13 mice per genotype per sex). (<i>A</i>) Results from Morris water maze test for spatial learning and memory. Graph of escape latency times. Points represent averages of daily trials. HD, hidden platform day (2 trials/session, 2 sessions/day); HD0, first trial on HD1; V, visible platform day (2 trials/session, 2 sessions/day). (<i>B–C</i>) Results from probe trials performed 72 h after the final hidden platform training (Probe 2). Data are presented as percent time spent in the target quadrant versus the average time spent in the other quadrants (<i>B</i>) and number of crossings over the original position of the target platform compared to the number of crossings over the equivalent platform positions in other quadrants (<i>C</i>). (<i>D–F</i>) Results from open field test for general locomotor activity. Data are presented as the total number of infrared beam breaks over the 15-minute testing period (<i>D</i>), ratio of activity in the center of the open field compared to activity in the center plus the periphery (<i>E</i>), and total number of rearings over a 15 minute period (<i>F</i>). CenT, total number of beam breaks in the center; TotT, total number of beam breaks in the center plus periphery. (<i>G</i>) Rotarod test for motor coordination. Rotarod was set at 16RPM and animals were tested during three independent trials, each lasting a maximum of 300 seconds. The average latency to fall is shown over all three trials. (<i>H–I</i>) Results from elevated plus maze test for anxiety. Maze consists of two open arms and two closed arms. The percent time spent by male and female WT and Arf4^+/− mice in the open (<i>H</i>) and closed (<i>I</i>) arms is shown. All data are mean±SEM. * p<0.05, *** p<0.001.