Autocrine signaling does not play a role for <i>Mtb</i>-mediated TF induction in macrophages.

<p>MDMs in 12-well plate were first infected with H37Rv or H37Ra (10 bacteria∶1 cell ratio) in 1 ml RPMI complete medium without antibiotic. After overnight, conditioned medium (CM) was collected and passed through 0.22 µm filter. MDMs in 48-well plate derived from the same donor were then incubated with 0.25 ml of the conditioned medium or live bacteria (10 bacteria∶1 cell ratio) in parallel for overnight. Thereafter, expression of TF activity at the cell surface was measured in factor X activation assay. ns, not statistically significant; * indicates significant difference compared to untreated control (P<0.005). Data are mean ± SEM ( n = 3–5).