JEV burden in serum and tissue samples.

<p>JEV burden in (<b>A</b>) serum, (<b>B</b>) spleen, (<b>C</b>) brain and (<b>D</b>) spinal cord of CCR5^+/+ and CCR5^−/− mice after i.v. infection with 10³ PFU of JEV. At the indicated time points, animals were sacrificed and the viral RNA content of serum and spleen samples were measured by real-time RT-PCR, while viral content in brain and spinal cord samples were measured by plaque titration. Data shown were constructed from 2 independent experiments. Each symbol represents an individual mouse, and geometric mean titers are indicated by horizontal lines. The lower limit of virus detection is indicated by the horizontal dotted line. Asterisks denote significant differences (<b>**</b>, <i>P</i><0.01).