Exercise training induced Nrf2 nuclear translocation and increased antioxidant levels.

<p>Immunofluorescence (IF) analysis using anti-Nrf2-ab (1∶200; v/v) showing nuclear translocation of Nrf2 in the hearts of young and old after MET (panel 1). Blue: nucleus (DAPI); Red: Nrf2-staining and Pink: merge of blue and red indicating nuclear localization of Nrf2. Panels 2–4 depict the expression of NQO1 (1∶400), HO1 (1∶150) and GSR (1∶200) in the cardiomyocytes of young and old mice after MET. The levels of these antioxidants were comparable in the cytosol of young and old (*p<0.05) cardiomyocytes after 6-weeks of exercise training. <b>^#</b>p<0.05 between basal and MET. IF images were obtained at 60X magnification.