MDS-MSC induce CD4+CD25+Foxp3+Tregs.

<p>(A) CD4+CD25-T cells were cultured with MDS-MSC or normal-MSC for 5 days, and CD4+ T cells were collected. The expression of CD25 and Foxp3 on CD4+ T cells was analyzed by FACS. Results are expressed as mean±SD of triplicates of 4 separate experiments. *P≤0.05. (B) CD4+T cells were cocultured with MDS-MSC generated CD4+CD25+Foxp3+Tregs or normal-MSC generated CD4+CD25+Foxp3+Tregs in the presence of PHA, and the T-lymphocyte proliferation was measured on day 5 by [3H]-thymidine incorporation. Results are expressed as mean±SD of triplicates of 4 separate experiments. *P≤0.05. (C) MDS-MSC generated CD4+CD25+Foxp3+Tregs or normal-MSC generated CD4+CD25+Foxp3+Tregs inhibited the response of allogeneic T-lymphocyte in a dose-dependent manner. Responder CD2+ T-lymphocyte were stimulated with PHA for 5 days with or without graded dosed of MDS-MSC generated CD4+CD25+Foxp3+Tregs or normal-MSC generated CD4+CD25+Foxp3+Tregs. Results are expressed as mean±SD of triplicates of 4 separate experiments. *p≤0.05. (D) CD4+CD25-T cells were cultured with high-risk MDS-MSC or low-risk MDS-MSC for 5 days, and CD4+ T cells were collected. The expression of CD25 and Foxp3 on CD4+ T cells was analyzed by FACS. Results are expressed as mean±SD of triplicates of 4 separate experiments. *P≤0.05.