Infectivity of AsPC-1 peritoneal tumors with the adenovirus displaying the selected peptide.

<p>(a) Infectivity of AsPC-1 peritoneal tumors with AdΔCAR-LucEGFP-PFW. The various amounts (1, 3, 10×10⁷ PFU) of adenovirus vectors (AdΔCAR-LucEGFP-PFW or AdΔCAR-LucEGFP) were intraperitoneally injected once into the mice with peritoneal dissemination of AsPC-1 cells (n = 3∼4). Two days after the injection, peritoneal tumors were harvested and the luciferase activity was measured. The graph presents the luciferase activity per mg of tumor tissue. AdΔCAR-LucEGFP is a replication-incompetent adenovirus vector ablated for CAR binding and expresses a fusion gene of luciferase and EGFP. In AdΔCAR-LucEGFP-PFW, a PFWSGAV peptide is inserted in HI-loop of fiber knob in AdΔCAR-LucEGFP. i.p.; intraperitoneal injection. (b) Infectivity of AsPC-1 peritoneal tumors with various adenovirus vectors. The 1×10⁸ PFU of adenovirus vectors (AdΔCAR-LucEGFP-PFW, AdΔCAR-LucEGFP-SYE, AdΔCAR-LucEGFP or Ad-LucEGFP) were intraperitoneally injected once into the mice with peritoneal dissemination of AsPC-1 cells (n = 5). Two days after the injection, peritoneal tumors were harvested and the luciferase activity was measured. In AdΔCAR-LucEGFP-SYE, a SYENFSA peptide is inserted in HI-loop of fiber knob in AdΔCAR-LucEGFP. Ad-LucEGFP is a replication-incompetent adenovirus vector with a wild type of fiber, and expresses a fusion gene of luciferase and EGFP. n.s.; not significant.