Roles of α-Syn in melanin synthesis in melanoma and dopaminergic neuronal cells.

<p>A375 melanoma cells (A) and dopaminergic neuronal SH-SY5Y and PC12 cells (C, D) with or without wt-α-Syn over-expression, SK-MEL-28 melanoma cells (B) with or without suppression of endogenous α-Syn, were exposed to UVB light (120 mJ/cm²) or non-UVB light and post-cultured for 24 h. The protein levels of α-Syn were determined by western blot assay (A, B, C, D, upper panel). Melanin content was determined spectrophotometrically at 415 nm using iMark Microplate Reader (A, B, C, D, lower panel). *: p<0.05; **: p<0.01; *** p<0.001 as compared to its non-UVB control (a), non-α-Syn control (b), or vector/<i>#3</i> siRNA control (c). Dox = doxycycline.