Xylanase thermostability and kinetics.

<p>After incubation at 50°C at 10-min interval from 10 to 30 min, the residual activity was assayed and expressed as a ratio relative to the un-incubated xylanase activity. The data were fitted with the equation <i>y = A^*e^-kt</i> (Origin, version 8.0), and thermostability (t_1/2) was calculated according to the decay function. The kinetics were assayed at T_opt and pH_opt conditions using birch-wood xylan at concentrations from 0 to 13 mg/ml. The data were fitted with the Hill function to calculate maximal activity (V_max) and K_m (Origin, version 8.0).