Inhibition of the effects of DHA metabolites in PC3 cells.

<p><b>A.</b> Cells not transfected or transfected with pcDNA3 or d/nPPARγ were challenged with 10 µM of the indicated metabolite for 24 h before assaying syndecan-1 mRNA. Values represent the mean ± SD (N = 3). Within a transfection group, bars labeled with the same letters are not significantly different from each other; bars labeled with different letters are significantly different from each other (one-way ANOVA, P<0.05). <b>B.</b> Cells untransfected or transfected with pcDNA3 or d/nPPARγ were challenged with 10 µM of the indicated metabolite for 3 days before assaying proliferation. Values represent the mean ± SD (N = 3). Within a metabolite group, bars labeled with the same letters are not significantly different from each other; bars labeled with different letters are significantly different from each other (one-way ANOVA, P<0.05). <b>C.</b> Cells were incubated with 0–1 µM of PPARγ antagonist, GW6692, for 30 min and with 10 µM of the indicated metabolite for 3 days before assaying proliferation. Results are presented as mean ± SEM (N = 3 independent experiments). Within a metabolite group, bars labeled with the same letters are not significantly different from each other; bars labeled with different letters are significantly different from each other (one-way ANOVA, P<0.05). <b>D.</b> Cells untransfected, transfected with control siRNA, or transfected with SDC-1 siRNA were challenged with 10 µM of the indicated metabolite for 3 days before assaying proliferation. Results are presented as mean ± SD (N = 4). Within a transfection group, bars labeled with the same letters are not significantly different from each other; bars labeled with different letters are significantly different from each other (one-way ANOVA, P<0.05).