Xylanase properties.

<p>Note: T_opt: optimal reaction temperature for activity, t_1/2: thermal in-activation half-life, after incubation at 50°C for different intervals, residual activity was determined and expressed as a ratio to the un-incubated enzyme, and data were fitted with Arrhenius function. The t_1/2 was calculated according to the decay function to indicate enzyme thermostability. Using the Student’s t-Test (<i>p</i><0.05), the XynΔC is more thermostable than the Xyn, and it is more thermostable than the XynΔN and the XynΔNC. The latter two enzymes are statistically similar thermostable. Kinetics was determined for birch-wood xylan at each enzyme pH_opt and T_opt conditions, and the data were fitted with Hill function to calculate V_max and K_m.