Propidium iodide analysis of apoptosis in hypoxic BMM treated with specific inhibitors.

<p>CSF-1-depleted BMM were incubated for 24 h in normoxic (A) and hypoxic (1% O2) conditions (B) and treated with DMSO vehicle. The hypoxic BMM were also treated with wortmannin (100 nM) (C), LY294002 (10 µM) (D), Akt VIII (10 µM) (E), rapamycin (60 nM) (F), U0126 (10 µM) (G), PD98059 (20 µM) (H), and SB203580 (10 µM) (I). DNA content that corresponds to apoptotic cells as defined by cells with <2N DNA (M1) is gated, and the percentage of total cells in M1 indicated. Data is representative of four separate experiments.