Apoptosis in hypoxic BMM in the presence of specific inhibitors.

<p>(A) The experimental protocol and analysis were as for the experiment in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045853#pone-0045853-g001" target="_blank">Figure 1</a>. The data for the inhibitors are “clustered” based on pathway specificity; wortmannin and LY294002 (PI3-K), Akt VIII (Akt), rapamycin (mTOR), UO126 and PD98059 (MEK1/2), and SB203580 (p38 MAPK). Data are mean values (± SEM) of % apoptosis from triplicate cultures from a representative experiment (n = 5). *, p<0.05 (Student's t-test) vs hypoxia/DMSO group; NS, not significant. (B) BMM were depleted of CSF-1 and left untreated (DMSO vehicle) or treated in triplicate cultures with Akt VIII (10 µM), under hypoxic or normoxic conditions for 2 days. Viable cell number was counted (trypan blue exclusion); t = 0, initial cell number. Data are mean values (± SEM) from a representative experiment (n = 2); *, p<0.05 (Student's t-test) vs corresponding untreated group.