Apoptosis in CSF-1-treated BMM in the presence of specific inhibitors.

<p>CSF-1-depleted BMM were incubated in normoxic conditions for 24 h in the presence of CSF-1 (640 U/mL). The cells were also treated with DMSO (vehicle), wortmannin (100 nM), LY294002 (10 µM), Akt VIII (10 µM), rapamycin (30 nM) and SB203580 (10 µM). DNA content was measured as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045853#pone-0045853-g001" target="_blank">Figure 1</a>. Data for the inhibitors are “clustered” as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0045853#pone-0045853-g002" target="_blank">Figure 2</a>. Data are mean values (±SEM) of % apoptosis from triplicate cultures from a representative experiment which was repeated at least three times. *, p<0.05 (Students' t test) vs DMSO group; NS, not significant.