Fluorescence intensity, lifetime mapping, and corresponding lifetime histogram of doxorubicin in HeLa cells.

<p>Cells were treated 5 µg/ml doxorubicin for 24 hrs. (A) Fluorescence intensity image; the contours of nuclear envelop and cell membrane are delineated with yellow and red dotted lines respectively, based on the phase contrast image. (B) Fluorescence lifetime image. Scale bar, 10 µm. (C) Corresponding lifetime histogram of nucleus and cytoplasm.