HuR binds to U-rich elements in 3′UTR of ΔNp63 transcript.

<p>(A) Schematic presentation of p63 3′UTR and probes used for REMSA. (B) REMSA was performed by mixing ³²P-labeled RNA probe a, b, or c with recombinant GST or GST-fused HuR. The bracket indicates RNA-protein complexes. (C) REMSA was performed by mixing ³²P-labeled RNA probe c1, c2, c3, or c4 with recombinant GST or GST-fused HuR. The bracket indicates RNA-protein complexes. (D) For competition assay, an excess amount of unlabeled p21 cold probe was added to a reaction mixture containing HuR and probe c. The bracket indicates RNA-protein complexes. (E) For supershift assay, 3 µg of control IgG or anti-HuR was added to a reaction mixture containing probe c with or without GST-HuR.