Cancer cells with high survivin expression are significantly more sensitive to FL118 treatment than non-cancerous cells, which have low or no survivin regardless of p53 status.

<p>Cells at about 50% confluence were treated with or without FL118 in a series of concentrations as shown (<b>a</b>, <b>c</b>) for 72 hours. Cell growth/viability were then determined using MTT assay and plotted as percentage cell viability curves. Alternatively, cells at 80–90% confluence were lysed and analyzed using Western blots for survivin expression (<b>b</b>, <b>d</b>). <b>a.</b> Cell viability curve for two cancer cell lines (SW620, HCT-8) versus two non-cancerous cell lines (AHDF, HGF) after FL118 treatment for 72 hours at the different concentrations as shown. <b>b.</b> Comparison of the expression of survivin among two cancer cell lines (SW620, HCT-8) versus two non-cancerous cell lines (AHDF, HGF). Actin was used as an internal control. <b>c.</b> Cell viability curve for additional two cancer cell lines (2008, FaDu) versus one non-cancerous cell line (AHDF) as a control after FL118 treatment for 72 hours at different concentrations as shown. <b>d.</b> Comparison of the expression of survivin in additional two cancer cell lines (2008, FaDu) versus one non-cancerous cell lines (AHDF) as a control. Actin was used as an internal control. The data on each data point shown in <b>a</b> and <b>c</b> are the mean ± SD derived from at least three independent assays in triplicate.