Effect of zanamivir and bacterial neuraminidase on the neutralization activity of saliva against influenza virus.

<p>Ten-fold serial dilutions of a saliva sample was mixed with an equal volume of A/Udorn/72 (<b>A</b>) and B/Johonnesburg/99 (<b>B</b>) virus suspensions (20,000 pfu/ml) containing zanamivir (+ Zanamivir, 500 nM) with or without <i>Vibrio cholerae</i> RDE (+ <i>V.c</i> RDE, 460 µunits/ml neuraminidase activity) and incubated at 37°C for 60 min. Survival infectivity (pfu) was determined by plaque assay.