Dose-dependent inhibition of mature Th17 cells by MSC.

<p>(A, C, E and G) Cell differentiation and (B, D, F and H) proliferation were assessed using T cells induced to differentiate into Th1 or Th17 cells in the absence or presence of MSC added at day 0 (CD4-Th1 or CD4-Th17, respectively) (A, B, C and D) or at day 4 of the differentiation process (Th1 or Th17, respectively), at two MSC:T ratios 1∶10 and 1∶100. (E, F, G and H). Results are expressed as the percentage of anti-CD3/CD28-induced proliferation of T cells which was assigned the value of 100%. We used the Cell Titer GloTM luminescence assay to quantify T cell proliferation and flow cytometry intracellular detection of IFN-γ (Th1) or RORγT and IL17 (Th17) for differentiation (A and C, representative dot plots). <i>P</i> values referred to the condition without MSC (no MSC).