Contribution of CD80 and CD86 to Interaction Forces and Cytokine Secretion.

<p>(<b>A</b>) Interaction forces; (<b>B</b>) IL-2 and (<b>C</b>) IFN-γ production of DC:T-cell or B:T-cell conjugates in the presence of blocking antibodies against CD80 and/or CD86. DCs or B-cells were pre-pulsed with Ova peptides (+Ova, 10 ng/ml) before antibody blocking. All force measurements were conducted with contact time of 3 min. *p<0.01; unpaired <i>t</i>-test. For each condition, OT-I T-cells were isolated from >3 independent experiments. αCD80: blocking antibody targeting CD80; αCD86: blocking antibody targeting CD86; αCD80/86: αCD80 and αCD86 simultaneously; IgG: isotype controls for both αCD80 and αCD86.